Recombinant Human CAMK1G/CaMKI gamma Protein (His & GST Tag)

Size: 50μg

Catalogue Number: PKSH030332-50

Citations, Manuals and MSDS Available upon request.

Abbreviation: CAMK1G, CaMKI gamma

Target Synonym: CLICK3, CLICKIII, dJ272L16.1, RP1-272L16.2, VWS1

UNIProt ID: Q96NX5

Target Species: Human

Expression Host: Baculovirus-Insect Cells

Fusion Tag: N-His & GST

Accession: Q96NX5-1

Background: Calmodulin-Dependent Protein Kinase (CaM Kinase) is a kind of protein phosphorylate multiple downstream targets. Concentration of cytosolic calcium functions as a second messenger that mediates a wide range of cellular responses. Calcium binds to calcium binding proteins (calmodulin/CaM) and stimulates the activity of a variety of enzymes, including CaM kinases referred to as CaM-kinases (CaMKs), such as CaMKI, CaMKII, CaMKIV and CaMKK. Calmodulin-dependent protein kinase CL3/CaMKI&gamma, is a memberane-anchored CaMK belonging to the CaM kinase family. Its C-terminal region is uniquely modified by two sequential lipidification steps: prenylation followed by a kinase-activity-regulated palmitoylation. These modifications are essential for CaMKI&gamma, membrane anchoring and targeting into detergent-resistant lipid microdomains in the dendrites. It has been found that CaMKI&gamma, critically contributed to BDNF-stimulated dendritic growth. Raft insertion of CaMKI&gamma, specifically promoted dendritogenesis of cortical neurons by acting upstream of RacGEF STEF and Rac, both present in lipid rafts. Thus, CaMKI&gamma, may represent a key element in the Ca2+-dependent and lipid-raft-delineated switch that turns on extrinsic activity-regulated dendrite formation in developing cortical neurons.

Sequence: Met 1-Met 476

Purity: > 85 % as determined by reducing SDS-PAGE.

Formulation: Supplied as sterile solution of 50mM Tris, 100mM NaCl, pH 8.0, 20% glycerol, 0.3mM DTT
Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
Please refer to the specific buffer information in the printed manual.

Reconstitution: Not Applicable

Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.

Calculated MW: 81 kDa

Observed MW: 75 kDa

Storage: Store at < -20°C, stable for 6 months. Please minimize freeze-thaw cycles.

Shipping: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.

Research Use Only